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KMID : 0368420030460040263
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Journal of Plant Biology
2003 Volume.46 No. 4 p.263 ~ p.270
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A Type-Ⅰ Chalcone Isomerase mRNA Is Highly Expressed in the Root Nodules of Elaeagnus umbellata
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Kim, Ho Bang
Oh, Chang Jae/Lee, Hyoungseok/An, Chung Sun
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Abstract
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We have used the hybridization-competition method to isolate EunOD-CHI from a root nodule cDNA library of Elae-agnug umbellata. This cDNA clone encodes chalcone isomerase (CHI) for a protein of 256 amino-acid residues and a mature molecular mass of 28 kDa. Multiple sequence alignment and phylogenetic analysis hate demonstrated that EuNOD-CHI can be classified as Type I. Moreover, northern hybridization shows that the EuNOD-CHI gene is highly expressed in root nodules, with levels increasing during nodule development, The highest level of expression is at 6 to 8 weeks after inoculation, decreasing thereafter. Genomic Southern hybridization also demonstrates that EuNOD-CHI has as many as two copies in the E, umbellata genome. Taken together with the previous resu1ts, we propose that the higher expression level of the EuNOD-CHI gene in root nodules is likely associated with this species´ defense mechanism against infection by Frantia.
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